Application
Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (detection by Goat anti-mouse IgG1 PE). Histograms: Red – Isotype mouse IgG1 control staining Black – standard anti-CD95 monoclonal antibody (DX2) Green – anti-CD95 (LT95) A – JURKAT human peripheral blood T cell leukemia cell line B – RAMOS human Burkitt lymphoma cell line C – CEM human leukemia cell line D – MOLT-4 human acute lymphoblastic T cell leukemia cell line| Product Name | Fas antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [LT95] to FasCD95, also known as FAS or APO-1, is a 36 kDa cell surface type I- membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. CD95 is a member of the TNF receptor family, which includes TNFR-1, TNFR-2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti-CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl-terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase-8 that together with the receptor form the death-inducing signaling complex (DISC). |
| Immunogen | Tissue / cell preparation (Human). (T cell lymphoma cells HUT-78). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | LT95 |
| Host Species | Mouse |
| Tested Applications | FACS |
| FACS: Use at an assay dependant concentration. Does not induce Fas mediated apoptosis in susceptible cells. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD95 FAS1 APO1 Apo-1 Fas Apo1 Fas 355 APT1 FASTM P25445 134637 APO-1 ALPS1A FAS TNFRSF6 APO 1 Apo 1 Fas |
|---|---|
| Target | Fas |
| Tissue Specificity | Human Fas (CD95/APO-1). |
| Cellular Localization | Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6). |
Application
Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (detection by Goat anti-mouse IgG1 PE). Histograms: Red – Isotype mouse IgG1 control staining Black – standard anti-CD95 monoclonal antibody (DX2) Green – anti-CD95 (LT95) A – JURKAT human peripheral blood T cell leukemia cell line B – RAMOS human Burkitt lymphoma cell line C – CEM human leukemia cell line D – MOLT-4 human acute lymphoblastic T cell leukemia cell line| Positive Control | Reacts strongly with IM9 and HUT-78 cell lines. Reacts moderately with Raji, Ramos, U266, Jurkat, BJAB and ML-1 cell lines. Reacts weakly with KG1a, U937, KM3 and TF-1 cell lines. Does not react with K562 and THP-1 cell lines. |
|---|---|
| Application Notes | FACS: Use at an assay dependant concentration. Does not induce Fas mediated apoptosis in susceptible cells. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
