Application
'Induction of apoptosis in various hematopoetic cell lines by use of anti-Fas antibodies. Suspensions of used cell lines were incubated with soluble (or without as control) anti-Fas antibodies. Induction of apoptosis was measured by Apoptosis Assay Kit - FITC. Histograms: Red (full) – untreated control cells Black – standard apoptosis-inducing anti-Fas antibody Green – anti-Fas (UT-1) A – JURKAT human peripheral blood T cell leukemia cell line B – TF-1 human bone marrow erythroleukemia cell line C – CEM human leukemia cell line D – MOLT-4 human acute lymphoblastic T cell leukemia cell line'| Product Name | Fas antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [UT-1] to FasCD95, also known as FAS or APO-1, is a 36 kDa cell surface type I- membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. CD95 is a member of the TNF receptor family, which includes TNFR-1, TNFR-2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti-CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl-terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase-8 that together with the receptor form the death-inducing signaling complex (DISC). |
| Immunogen | T cell lymphoma cells HUT-78. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | UT-1 |
| Host Species | Mouse |
| Tested Applications | FACS |
| FACS: Use at an assay dependent dilution. FuncS: Use at an assay dependent dilution. The antibody induces apoptosis of CD95 expressing cell lines. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | CD95 FAS1 APO1 Apo-1 Fas Apo1 Fas 355 APT1 FASTM P25445 134637 ALPS1A APO-1 FAS TNFRSF6 APO 1 Apo 1 Fas |
|---|---|
| Target | Fas |
| Cellular Localization | Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6). |
Application
'Induction of apoptosis in various hematopoetic cell lines by use of anti-Fas antibodies. Suspensions of used cell lines were incubated with soluble (or without as control) anti-Fas antibodies. Induction of apoptosis was measured by Apoptosis Assay Kit - FITC. Histograms: Red (full) – untreated control cells Black – standard apoptosis-inducing anti-Fas antibody Green – anti-Fas (UT-1) A – JURKAT human peripheral blood T cell leukemia cell line B – TF-1 human bone marrow erythroleukemia cell line C – CEM human leukemia cell line D – MOLT-4 human acute lymphoblastic T cell leukemia cell line'| Application Notes | FACS: Use at an assay dependent dilution. FuncS: Use at an assay dependent dilution. The antibody induces apoptosis of CD95 expressing cell lines. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
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| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: none; Constituents: PBS. pH 7.4 |
