Application
Western blot analysis of human Fas expression in human Fas transfected cells Lane 1: anti-human Fas (ZB4) Lane 2: anti-human Fas (UB2) Lane 3: anti-human Fas (CH11).| Product Name | Fas antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [ZB4] to FasCD95, also known as FAS or APO-1, is a 36 kDa cell surface type I- membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. CD95 is a member of the TNF receptor family, which includes TNFR-1, TNFR-2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti-CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl-terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase-8 that together with the receptor form the death-inducing signaling complex (DISC). |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | ZB4 |
| Host Species | Mouse |
| Tested Applications | BlockingFACSIHCWB |
| FACS: Use at a concentration of 5 - 20 μg/ml. FuncS: Use at a concentration of 500 ng/ml to block the apoptotic activity other anti-CD95 antibodies. IHC: Use at an assay dependent dilution. WB: Use at a concentration of 5 μg/ml. Predicted molecular weight: 44 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | CD95 FAS1 APO1 Apo-1 Fas Apo1 Fas 355 APT1 FASTM P25445 134637 APO-1 ALPS1A FAS TNFRSF6 APO 1 Apo 1 Fas |
|---|---|
| Target | Fas |
| Tissue Specificity | This antibody has been demonstrated to block the apoptotic activity of other anti-CD95 antibodies that are known to induce apoptosis. |
| Cellular Localization | Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6). |
Application
Western blot analysis of human Fas expression in human Fas transfected cells Lane 1: anti-human Fas (ZB4) Lane 2: anti-human Fas (UB2) Lane 3: anti-human Fas (CH11).| Application Notes | FACS: Use at a concentration of 5 - 20 μg/ml. FuncS: Use at a concentration of 500 ng/ml to block the apoptotic activity other anti-CD95 antibodies. IHC: Use at an assay dependent dilution. WB: Use at a concentration of 5 μg/ml. Predicted molecular weight: 44 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: 10mg/ml Sucrose, PBS. pH 7.2 |
