Application
Sandwich ELISA analysis of human Fas Ligand binding using Mouse anti Human Fas Ligand as a capture reagent and Mouse anti Human Fas Ligand as a detection reagent with purified human Fas Ligand as antigen for the generation of a standard curve. Plasma (orange) sample is displayed at 1:2 dilution.Application
FACS analysis of permeabilised Jurkat cells using Fas Ligand antibody [14C2].| Product Name | Fas Ligand antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [14C2] to Fas LigandThe protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with this ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 14C2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIP |
| Flow Cytometry: 1/25 - 1/50 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD95-L ALPS1B FASL CD178 APTL FASLG CD95L TNFSF6 APT1LG1 |
|---|---|
| Target | CD178 |
| Tissue Specificity | This antibody recognizes the human CD178, also known as Tumor necrosis factor ligand superfamily member 6, Fas ligand (FasL), Apoptosis antigen ligand or CD95 ligand. CD178 is a 281 amino acid, a ~40kDa single pass type-II transmembrane glycoprotein beari |
Application
Sandwich ELISA analysis of human Fas Ligand binding using Mouse anti Human Fas Ligand as a capture reagent and Mouse anti Human Fas Ligand as a detection reagent with purified human Fas Ligand as antigen for the generation of a standard curve. Plasma (orange) sample is displayed at 1:2 dilution.
Application
FACS analysis of permeabilised Jurkat cells using Fas Ligand antibody [14C2].| Application Notes | Flow Cytometry: 1/25 - 1/50 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
