Application
Western blot Analysis of FasL expression in 293 Cell Lysate.| Product Name | Fas Ligand antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [I-6060] to Fas LigandFas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). FasL is a tumor necrosis factor (TNF)-related type II membrane protein and is expressed in various tissue and cells. Both Fas and FasL are thought to play an important role in the apoptosis processes that takes place during T cell development. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | I-6060 |
| Host Species | Mouse |
| Tested Applications | IHCIPWB |
| Western blotting (1-4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control and an approximately 40 kDa band should be detected by Western blotting.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FASL CD95L APT1LG1 CD178 APTL FASLG TNFSF6 |
|---|---|
| Target | Fas Ligand |
| Tissue Specificity | This antibody is specific for Fas Ligand. |
| Cellular Localization | May be released into the extracellular fluid, probably by cleavage form the cell surface. |
Application
Western blot Analysis of FasL expression in 293 Cell Lysate.| Application Notes | Western blotting (1-4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control and an approximately 40 kDa band should be detected by Western blotting.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% thimerosal |
