Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000Application
FAST antibody detects FAST protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FAST protein stained by FAST antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunofluorescence analysis of methanol-fixed HeLa, using FAST antibody at 1:200 dilution.Application
FAST antibody detects FASTK protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE FAST antibody dilution: 1:1000Application
FAST antibody detects FASTK protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5 % SDS-PAGE FAST antibody dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using FAST antibody at 1:100 dilution.| Product Name | FAST antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FAST (Fas-activated serine/threonine kinase)The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human FAST. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, Chimpanzee, BovineDog (95%), Pig (90%), Chimpanzee (99%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FAST antibody FASTK antibody FAST kinase antibody fas-activated serine/threonine kinase antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000
Application
FAST antibody detects FAST protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FAST protein stained by FAST antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using FAST antibody at 1:200 dilution.
Application
FAST antibody detects FASTK protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE FAST antibody dilution: 1:1000
Application
FAST antibody detects FASTK protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5 % SDS-PAGE FAST antibody dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using FAST antibody at 1:100 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , NIH-3T3 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
