Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325] diluted at 1:500.Application
Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:5000Application
Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:500Application
Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:1000| Product Name | Fatty Acid Synthase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Fatty Acid Synthase (fatty acid synthase)The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT325 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | fatty acid synthase FAS FASN OA-519 SDR27X1 |
|---|---|
| Cellular Localization | Cytoplasm , Melanosome |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325] diluted at 1:500.
Application
Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:5000
Application
Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:500
Application
Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:1000| Positive Control | 293T , Neuro2A , PC-12 , A431 , GL261 , Rat-2 , HepG2 , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
Data sheet for OM252698