Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using FBL4 antibody at 1:100 dilution.Application
FBL4 antibody [N1C1] detects FBL4 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human FBXL4-transfected 293T cells 7.5 % SDS-PAGE FBL4 antibody [N1C1] dilution: 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FBL4 antibody at 1:200 dilution.| Product Name | FBL4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FBL4 (F-box and leucine-rich repeat protein 4)This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 9 tandem leucine-rich repeats. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FBL4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Pig, Rhesus Monkey, BovineMouse (91%), Pig (92%), Rhesus Monkey (96%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FBL4 antibody FBL5 antibody FBXL4 antibody F-box protein FBL4 antibody F-box protein FBL4/FBL5 antibody F-box/LRR-repeat protein 4 antibody F-box and leucine-rich repeat protein 4 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using FBL4 antibody at 1:100 dilution.
Application
FBL4 antibody [N1C1] detects FBL4 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human FBXL4-transfected 293T cells 7.5 % SDS-PAGE FBL4 antibody [N1C1] dilution: 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FBL4 antibody at 1:200 dilution.| Positive Control | 293T , human FBXL4-transfected 293T cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
