Application
ICC/IF ImageFBL6 antibody [C3], C-term detects FBXL6 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FBXL6 protein stained by FBL6 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
IHC-P ImageImmunohistochemical analysis of paraffin-embedded human thyroid cancer, using FBL6 antibody at 1:100 dilution.Application
WB ImageSample(30 μg of whole cell lysate) A:HeLa S3 10% SDS PAGE diluted at 1:1500| Product Name | FBL6 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FBL6 (F-box and leucine-rich repeat protein 6)This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FBL6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FBL6 antibody FBL6A antibody PP14630 antibody FBXL6 antibody F-box protein Fbl6 antibody F-box/LRR-repeat protein 6 antibody F-box and leucine-rich repeat protein 6 antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (2) |
Application
ICC/IF ImageFBL6 antibody [C3], C-term detects FBXL6 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FBXL6 protein stained by FBL6 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
IHC-P ImageImmunohistochemical analysis of paraffin-embedded human thyroid cancer, using FBL6 antibody at 1:100 dilution.
Application
WB ImageSample(30 μg of whole cell lysate) A:HeLa S3 10% SDS PAGE diluted at 1:1500| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
