Rabbit Polyclonal antibody to FBLIM1 (filamin binding LIM protein 1)This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human FBLIM1. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-PWB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
CAL antibody DKFZp434G171 antibody FBLP-1 antibody FBLP1 antibody FBLIM1 antibody migfilin antibody filamin-binding LIM protein 1 antibody mitogen-inducible 2 interacting protein antibody mitogen-inducible 2-interacting protein antibody MIG2-inter
Molecular Weight(MW)
41 kDa (note) (3)
Application
Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft, using FBLIM1 antibody at 1:250 dilution.
Positive Control
HCT116
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.