Application
Sample (30 µg of whole cell lysate) A: A549 B: HeLa 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse brain 5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FBXL10 antibody [C3], C-term detects FBXL10 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. FBXL10 antibody [C3], C-term diluted at 1:500.WB
(A) Western blotting and (B) semi-quantification of protein expression levels.WB
(A) Western blotting and (B) semi-quantification of protein expression levels.| Product Name | FBXL10 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FBXL10 (lysine-specific demethylase 2B)This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FBXL10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | CXXC2 antibody FBXL10 antibody Fbl10 antibody JHDM1B antibody PCCX2 antibody KDM2B antibody F-box and leucine-rich repeat protein 10 antibody CXXC-type zinc finger protein 2 antibody jmjC domain-containing histone demethylation protein 1B antibody |
|---|---|
| Molecular Weight(MW) | 153 kDa (note) |
Application
Sample (30 µg of whole cell lysate) A: A549 B: HeLa 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FBXL10 antibody [C3], C-term detects FBXL10 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. FBXL10 antibody [C3], C-term diluted at 1:500.
WB
(A) Western blotting and (B) semi-quantification of protein expression levels.
WB
(A) Western blotting and (B) semi-quantification of protein expression levels.| Positive Control | A549 , HeLa , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
