Application
Sample (30 ug of whole cell lysate) A: Hela B: Raji 10% SDS PAGE diluted at 1:2000Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using FBXL2 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using FBXL2 antibody at 1:200 dilution.| Product Name | FBXL2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FBXL2 (F-box and leucine-rich repeat protein 2)This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human FBXL2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp564P0622 antibody FBL2 antibody FBL3 antibody FBXL2 antibody F-box protein containing leucine-rich repeats antibody F-box/LRR-repeat protein 2 antibody F-box protein FBL2/FBL3 antibody F-box and leucine-rich repeat protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: Hela B: Raji 10% SDS PAGE diluted at 1:2000
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using FBXL2 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using FBXL2 antibody at 1:200 dilution.| Positive Control | A431 , HeLa , HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
