Application
Western blot of Fe65-affinity pulldown materials from mouse brain lysate using Lane 1 is an immunopurified sample from a full-length Fe65 Knockout mouse. Lane 2 is an immunopurified sample from an Fe65 wild type mouse.| Product Name | Fe65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Fe65The major transcript of the Fe65 gene is a neuron-specific mRNA that encodes a nuclear protein whose amino-terminal domain strongly activates the transcription of reporter genes. Fe65 has been implicated in a regulatory and cell signaling mechanism because it contains two different motifs involved in protein binding; a WW domain (a variant of Src homology 3 domains) and a phosphotyrosine interaction domain (PID). Interestingly, the PID domain binds to the same motif present in the conserved cytoplasmic domains of the beta PP and beta PP-like proteins. RNA analyses revealed that Fe65 is predominantly expressed in the brain and in the regions most affected by Alzheimer’s disease-associated neuropathology. |
| Immunogen | Synthetic Peptide: P(352) Q E E E K L S Q R N A N P G I K C(369) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC/IF: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FE65, 11785, Rir, Apbb1 |
|---|---|
| Target | Fe65 |
Application
Western blot of Fe65-affinity pulldown materials from mouse brain lysate using Lane 1 is an immunopurified sample from a full-length Fe65 Knockout mouse. Lane 2 is an immunopurified sample from an Fe65 wild type mouse.| Application Notes | ICC/IF: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
