Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:5000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FEM1C antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | FEM1C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FEM1C (fem-1 homolog c (C. elegans))Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human FEM1C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (86%), Xenopus laevis (91%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EUROIMAGE686608 antibody, EUROIMAGE783647 antibody, FEM1A antibody, KIAA1785 antibody, FEM1C antibody, FEM1-gamma antibody, protein fem-1 homolog C antibody, fem-1 homolog c (C. elegans) antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:5000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FEM1C antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
