Application
Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.Application
Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.Application
Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.Application
Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.Application
Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.| Product Name | Ferritin Heavy Chain antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Ferritin Heavy Chain (ferritin, heavy polypeptide 1)This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Ferritin Heavy Chain. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Cat, Pig, Chicken, Sheep, Rhesus Monkey, Bovine, Guinea pigDog (96%), Cat (96%), Pig (87%), Chicken (93%), Sheep (93%), Rhesus Monkey (99%), Bovine (92%), Guinea pig (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FHC antibody, FTH antibody, FTHL6 antibody, MGC104426 antibody, PIG15 antibody, PLIF antibody, FTH1 antibody, ferritin heavy chain antibody, apoferritin antibody, cell proliferation-inducing gene 15 protein antibody, ferritin H subunit antibody, placenta i |
|---|---|
| Target | Ferritin Heavy Chain |
| Molecular Weight(MW) | 21 kDa (note) |
Application
Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.
Application
Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.
Application
Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.
Application
Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.
Application
Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.| Positive Control | human liver , mouse liver , rat liver , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
