Application
Fetuin B antibody detects Fetuin B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin B antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Fetuin B antibody at 1:100 dilution.Application
Fetuin B antibody detects Fetuin B protein at cytoplasm by immunofluorescent analysis. Sample: SK-N_SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fetuin B protein stained by Fetuin B antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of Fetuin B protein from SK-N-SH whole cell extracts using 5 μg of Fetuin B antibody . Western blot analysis was performed using Fetuin B antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | Fetuin B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Fetuin B (fetuin B)The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fetuin B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 16G2 antibody, Gugu antibody, IRL685 antibody, FETUB antibody, fetuin-like protein IRL685 antibody, fetuin-B antibody, fetuin B antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Fetuin B antibody detects Fetuin B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin B antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Fetuin B antibody at 1:100 dilution.
Application
Fetuin B antibody detects Fetuin B protein at cytoplasm by immunofluorescent analysis. Sample: SK-N_SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fetuin B protein stained by Fetuin B antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of Fetuin B protein from SK-N-SH whole cell extracts using 5 μg of Fetuin B antibody . Western blot analysis was performed using Fetuin B antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse plasma , rat plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
