Application
Western blot analysis of FGF1 (arrow) using rabbit polyclonal FGF1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGF1 gene (Lane 2)Application
Confocal immunofluorescent analysis of FGF1 Antibody (N-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with FGF1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | FGF1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FGF1 (N-term)The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq] |
| Immunogen | The immunogen corresponding to a region within amino acids 5 and 30 of human FGF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| For WB: Use at a dilution of 1:1000. | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | HBGF1, ECGF-beta, Beta-endothelial cell growth factor, FGF1, aFGF, Betaendothelial cell growth factor, ECGFbeta, FGFA, Heparin-binding growth factor 1 [Precursor], HBGF-1, Heparinbinding growth factor 1 [Precursor], Acidic fibroblast growth fac, Heparinbin |
|---|---|
| Target | FGF1 |
| Molecular Weight(MW) | 17.46 kDa (note) |
Application
Western blot analysis of FGF1 (arrow) using rabbit polyclonal FGF1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGF1 gene (Lane 2)
Application
Confocal immunofluorescent analysis of FGF1 Antibody (N-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with FGF1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | For WB: Use at a dilution of 1:1000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
