Application
Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
FGFR2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane). This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).Application
FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane). This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary FGFR2 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinomaApplication
Confocal immunofluorescent analysis of FGFR2 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
FGFR2 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term). was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Product Name | FGFR2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FGFR2Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families). |
| Immunogen | The immunogen corresponding to a region within amino acids 22 and 51 of Human FGFR2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| For WB: Use at a dilution of 1:1000. | |
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | JWS, ECT1, CFD1, TK25, BFR1, CEK3, 101200, TK14, BEK, KGFR, 2263, K-SAM, BFR-1, P21802, FGFR2, KSAM, K SAM, BFR 1 |
|---|---|
| Target | FGFR2 |
| Cellular Localization | Plasma membrane |
Application
Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
FGFR2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane). This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).
Application
FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane). This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary FGFR2 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma
Application
Confocal immunofluorescent analysis of FGFR2 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
FGFR2 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term). was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Positive Control | Jurkat cell lysate. |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1000. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline containing 0.09% sodium azide |
