Application
Western blot analysis of anti-FGFR2 Antibody (N-term R22) in Hela cell line lysates (35ug/lane). FGFR2(arrow) was detected using the purified Pab.Application
FGFR2 Antibody (N-term R22) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of FGFR2 Antibody (N-term R22) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | FGFR2 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FGFR2 (N-term R22)The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq] |
| Immunogen | The immunogen corresponding to a region within amino acids 3 and 37 of human FGFR2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFWB |
| We recommend the following starting dilutions: For WB: Use at a dilution of 1:1000. For ELISA: Use an assay dependent dilution. For ICC/IF: Use at a dilution of 1:10-1:50. For FACS: Use at a dilution of 1:10-1:50. Optimal dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | FGFR2 antibody, fibroblast growth factor receptor 2 antibody, BEK antibody, JWS antibody, BBDS antibody, CEK3 antibody, CFD1 antibody, ECT1 antibody, KGFR antibody, TK14 antibody, TK25 antibody, BFR-1 antibody, CD332 antibody, K-SAM antibody |
|---|---|
| Target | FGFR2 |
| Molecular Weight(MW) | 92.025 kDa (note) |
Application
Western blot analysis of anti-FGFR2 Antibody (N-term R22) in Hela cell line lysates (35ug/lane). FGFR2(arrow) was detected using the purified Pab.
Application
FGFR2 Antibody (N-term R22) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of FGFR2 Antibody (N-term R22) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | We recommend the following starting dilutions: For WB: Use at a dilution of 1:1000. For ELISA: Use an assay dependent dilution. For ICC/IF: Use at a dilution of 1:10-1:50. For FACS: Use at a dilution of 1:10-1:50. Optimal dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
