Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:500Application
FGR antibody [C1C3] detects FGR protein at cytoplasm by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FGR protein stained by FGR antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | FGR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FGR (Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog)This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FGR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (89%), Rat (89%), Rhesus Monkey (97%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ43153 antibody MGC75096 antibody SRC2 antibody c-fgr antibody c-src2 antibody p55-Fgr antibody p55c-fgr antibody p58c-fgr antibody FGR antibody c-src-2 proto-oncogene antibody tyrosine-protein kinase Fgr antibody proto-oncogene c-Fgr antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:500
Application
FGR antibody [C1C3] detects FGR protein at cytoplasm by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FGR protein stained by FGR antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
