Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000Application
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FHL1 antibody diluted at 1:1000.| Product Name | FHL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FHL1 (four and a half LIM domains 1)LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FHL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, Sheep, BovinePig (100%), Sheep (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FHL1A antibody FLH1A antibody KYOT antibody MGC111107 antibody SLIM1 antibody SLIMMER antibody XMPMA antibody FHL1 antibody four-and-a-half Lin11 Isl-1 and Mec-3 domains 1 antibody skeletal muscle LIM-protein1 antibody four and a half L |
|---|---|
| Target | FHL1 |
| Molecular Weight(MW) | 36 kDa (note) (6) |
| Tissue Specificity | Based on sequence homology, is expected to recognize isoform 1 (FHL1A), isoform 4, and isoform 5, but not isoform 2 (FHL1B) and isoform 3 (FHL1C) of human FHL1 (Uniprot Q13642). |
| Cellular Localization | Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Nucleus , Isoform 2: Nucleus , Cytoplasm , cytosol |
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000
Application
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FHL1 antibody diluted at 1:1000.| Positive Control | HeLa , mouse muscle , mouse lung |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
