Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using FHL5 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: Raji 12 % SDS PAGE diluted at 1:1000| Product Name | FHL5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FHL5 (four and a half LIM domains 5)The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FHL5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chimpanzee, BovineMouse (89%), Rat (90%), Pig (92%), Chimpanzee (98%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACT antibody FLJ33049 antibody KIAA0776 antibody RP3-393D12.2 antibody dJ393D12.2 antibody FHL5 antibody FHL-5 antibody LIM protein ACT antibody activator of cAMP-responsive element modulator (CREM) in testis antibody activator of CREM in testis a |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using FHL5 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: Raji 12 % SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
