| Product Name | Fibrillin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [11C1.3] to Fibrillin 1Fibrillin 1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. Fibrillin 1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in Fibrillin 2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. |
| Immunogen | Microfibrils from zonular apparatus of bovine eye. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 11C1.3 |
| Host Species | Mouse |
| Tested Applications | EMICC/IFIHC-FWB |
| Electron Microscopy / Immunofluorescence / Immunohistochemistry: assay dependent. Western blot: use at a concentration of 1 - 2μg/ml for 2 hours at RT. By Western blot this detects a band of ~ 350 kDa, which corresponds to the predicted molecular weight of Fibrillin 1. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseBovine |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | FBN 281154 WMS MFS1 OCTD Fibrillin1 FBN1 MASS 129600 2200 fibrillin Fibrillin 1 P35555 SGS |
|---|---|
| Target | Fibrillin 1 |
| Cellular Localization | Extracellular microfibrils. |
| Light Chain Type | kappa |
| Positive Control | Normal skin fibroblasts |
|---|---|
| Application Notes | Electron Microscopy / Immunofluorescence / Immunohistochemistry: assay dependent. Western blot: use at a concentration of 1 - 2μg/ml for 2 hours at RT. By Western blot this detects a band of ~ 350 kDa, which corresponds to the predicted molecular weight of Fibrillin 1. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
