Application
Fibrillarin antibody detects Fibrillarin protein at nucleolus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Fibrillarin antibody diluted at 1:500.Application
Fibrillarin antibody detects Fibrillarin protein by western blot analysis. whole cell lysate (30 μg) and nuclear extract (30 μg) from IMR32 cells 10 % SDS-PAGE Fibrillarin antibody dilution: 1:500Application
Fibrillarin antibody detects Fibrillarin protein by western blot analysis. whole cell lysate (30 μg) and nuclear extract (30 μg) from SK-N-SH cells 10 % SDS-PAGE Fibrillarin antibody dilution: 1:500Application
Fibrillarin antibody detects Fibrillarin protein by western blot analysis. whole cell lysate (30 μg) and nuclear extract (30 μg) from Jurkat cells 10 % SDS-PAGE Fibrillarin antibody dilution: 1:500Application
Fibrillarin antibody detects Fibrillarin protein by western blot analysis. whole cell lysate (30 μg) and nuclear extract(30 μg) from A549 cells 10 % SDS-PAGE Fibrillarin antibody dilution: 1:500Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrillarin antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using Fibrillarin antibody at 1:100 dilution.Application
Fibrillarin antibody detects FBL protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract 12 % SDS-PAGE Fibrillarin antibody dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: 293T B: H1299 C: Molt-4 12% SDS PAGE diluted at 1:1000Application
Fibrillarin antibody detects FBL protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12 % SDS-PAGE Fibrillarin antibody dilution: 1:500| Product Name | Fibrillarin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Fibrillarin (fibrillarin)This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, BovineZebrafish (89%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FIB antibody FLRN antibody RNU3IP1 antibody FBL antibody 34 kDa nucleolar scleroderma antigen antibody RNA U3 small nucleolar interacting protein 1 antibody rRNA 2'-O-methyltransferase fibrillarin antibody 34-kD nucleolar scleroderma antigen antibo |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (2) |
| Cellular Localization | Nucleus , nucleolus |
Application
Fibrillarin antibody detects Fibrillarin protein at nucleolus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Fibrillarin antibody diluted at 1:500.
Application
Fibrillarin antibody detects Fibrillarin protein by western blot analysis. whole cell lysate (30 μg) and nuclear extract (30 μg) from IMR32 cells 10 % SDS-PAGE Fibrillarin antibody dilution: 1:500
Application
Fibrillarin antibody detects Fibrillarin protein by western blot analysis. whole cell lysate (30 μg) and nuclear extract (30 μg) from SK-N-SH cells 10 % SDS-PAGE Fibrillarin antibody dilution: 1:500
Application
Fibrillarin antibody detects Fibrillarin protein by western blot analysis. whole cell lysate (30 μg) and nuclear extract (30 μg) from Jurkat cells 10 % SDS-PAGE Fibrillarin antibody dilution: 1:500
Application
Fibrillarin antibody detects Fibrillarin protein by western blot analysis. whole cell lysate (30 μg) and nuclear extract(30 μg) from A549 cells 10 % SDS-PAGE Fibrillarin antibody dilution: 1:500
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrillarin antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using Fibrillarin antibody at 1:100 dilution.
Application
Fibrillarin antibody detects FBL protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract 12 % SDS-PAGE Fibrillarin antibody dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: 293T B: H1299 C: Molt-4 12% SDS PAGE diluted at 1:1000
Application
Fibrillarin antibody detects FBL protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12 % SDS-PAGE Fibrillarin antibody dilution: 1:500| Positive Control | 293T , H1299 , Molt-4 , Neuro2A , PC-12 , A549 , A549 nuclear extract , Jurkat , Jurkat nuclear extract , SK-N-SH , SK-N-SH nuclear extract , IMR32 , IMR32 nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
