Mouse monoclonal antibody [EP5] to Fibronectin (Matrix)This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Immunogen
Native human fibronectin (matrix)
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
EP5
Host Species
Mouse
Tested Applications
FACSICC/IFIHC-FIHC-P
Immunohistology - Paraffin: 1/25 - 1/100 Optimal dilution / concentration should be determined by the end user.:
Recognises fibronectin in human and mouse tissues, specifically connective tissues and vessels. No reactivity with the soluble dimeric form of fibronectin (plasma fibronectin), but highly reactive with matrix fibronectin.
Application
Application
IHC-P analysis of human kidney tissue using Fibronectin (Matrix) antibody. Antigen retrieval using citrate buffer.
Application Notes
Immunohistology - Paraffin: 1/25 - 1/100 Optimal dilution / concentration should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.