Application
Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 μg of Fibronectin antibody [N1N2], N-term 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin antibody at 1:200 dilution.Application
Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term dilution: 1:500.| Product Name | Fibronectin antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Fibronectin (fibronectin 1)This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Fibronectin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, BovineRat (92%), Chicken (88%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CIG antibody DKFZp686F10164 antibody DKFZp686H0342 antibody DKFZp686I1370 antibody DKFZp686O13149 antibody ED-B antibody FINC antibody FN antibody FNZ antibody GFND antibody GFND2 antibody LETS antibody MSF antibody FN1 antibody migration-sti |
|---|---|
| Target | Fibronectin |
| Molecular Weight(MW) | 263 kDa (note) (9) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 μg of Fibronectin antibody [N1N2], N-term 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin antibody at 1:200 dilution.
Application
Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term dilution: 1:500.| Positive Control | HepG2 , HepG2(10 mM Nicotinamide and 0.4 mM Trichostatin A treatment for 48 hr) , Mouse plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
