Application
FIS1 antibody detects FIS1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TTC11 antibody dilution: 1:500.Application
FIS1 antibody detects FIS1 protein by western blot analysis. A. 50 µg rat brain lysate/extract 15% SDS-PAGE FIS1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 antibody diluted at 1:2000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1 antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: Raji 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | FIS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FIS1 (fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae))The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM] |
| Immunogen | Full length human FIS1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TTC11 antibody TPR repeat protein 11 antibody hFis1 antibody tetratricopeptide repeat domain 11 antibody mitochondrial fission 1 protein antibody FIS1 homolog antibody H_NH0132A01.6 antibody tetratricopeptide repeat protein 11 antibody fiss |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein , Peroxisome membrane; Single-pass membrane protein |
Application
FIS1 antibody detects FIS1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TTC11 antibody dilution: 1:500.
Application
FIS1 antibody detects FIS1 protein by western blot analysis. A. 50 µg rat brain lysate/extract 15% SDS-PAGE FIS1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 antibody diluted at 1:2000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1 antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: Raji 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Raji , 293T , A431 , HeLa , HepG2 , mouse liver , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
