Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FKBP4 antibody diluted at 1:3000.Application
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract 10 % SDS-PAGE FKBP4 antibody dilution: 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using FKBP52 antibody at 1:200 dilution.Application
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 10 % SDS-PAGE FKBP4 antibody dilution: 1:1000Application
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 10 % SDS-PAGE FKBP4 antibody dilution: 1:1000| Product Name | FKBP4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FKBP4 (FK506 binding protein 4, 59kDa)The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FKBP4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rabbit, Chicken, Rhesus Monkey, BovinePig (97%), Rabbit (93%), Chicken (85%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FKBP51 antibody FKBP52 antibody FKBP59 antibody HBI antibody Hsp56 antibody PPIase antibody p52 antibody FKBP4 antibody FK506-binding protein 4 (59kD) antibody T-cell FK506-binding protein 59kD antibody peptidylprolyl cis-trans isomerase antibod |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FKBP4 antibody diluted at 1:3000.
Application
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract 10 % SDS-PAGE FKBP4 antibody dilution: 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using FKBP52 antibody at 1:200 dilution.
Application
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 10 % SDS-PAGE FKBP4 antibody dilution: 1:1000
Application
FKBP4 antibody detects FKBP4 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 10 % SDS-PAGE FKBP4 antibody dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , GL261 , C8D30 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
