Rabbit Polyclonal antibody to Flh (floating head)Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Immunogen
Recombinant protein encompassing a sequence within the center region of zebrafish Flh. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-W
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Zebra Fish
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
flh floating head Znot
Target
Flh
Application
Application
Flh antibody detects Flh protein on zebrafish by whole mount immunohistochemical analysis. Sample: 24 hours-post-fertilization zebrafish embryo. Flh antibody dilution: 1:100.
Positive Control
24 hours-post-fertilization zebrafish embryo
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.