Application
FLI1 antibody detects FLI1 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract 10% SDS-PAGE FLI1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FLI1 antibody detects FLI1 protein by western blot analysis. A. 50 µg rat kidney lysate/extract 10% SDS-PAGE FLI1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using FLI1 antibody at 1:500 dilution.Application
FLI1 antibody detects FLI1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLI1 protein stained by FLI1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
FLI1 antibody immunoprecipitates FLI1 protein in IP experiments. IP samples: Raji whole cell extract A. 40 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 μg FLI1 antibody 10 % SDS-PAGE The immunoprecipitated FLI1 protein was detected by FLI1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | FLI1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FLI1 (Friend leukemia virus integration 1)Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, BovineXenopus laevis (83%), Chicken (91%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EWSR2 antibody SIC-1 antibody FLI1 antibody transcription factor ERGB antibody Friend leukemia integration 1 transcription factor antibody proto-oncogene Fli-1 antibody Friend leukemia virus integration 1 antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) |
| Cellular Localization | Nucleus |
Application
FLI1 antibody detects FLI1 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract 10% SDS-PAGE FLI1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FLI1 antibody detects FLI1 protein by western blot analysis. A. 50 µg rat kidney lysate/extract 10% SDS-PAGE FLI1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using FLI1 antibody at 1:500 dilution.
Application
FLI1 antibody detects FLI1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLI1 protein stained by FLI1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
FLI1 antibody immunoprecipitates FLI1 protein in IP experiments. IP samples: Raji whole cell extract A. 40 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 μg FLI1 antibody 10 % SDS-PAGE The immunoprecipitated FLI1 protein was detected by FLI1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , Raji , NCI-H929 , BCL-1 , rat kidney |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
