Application
Western blot analysis of FLI1 antibody (Center) (Cat.# AP6514c) in mouse lung tissue lysates(35ug/lane). FLI1 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of FLI1 Antibody (Center)(Cat#AP6514c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Product Name | FLI1 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FLI1 (Internal)Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. |
| Immunogen | The immunogen corresponding to a region within amino acids 299 and 328 of human FLI1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Recommended Starting Dilutions: WB: Use at 1:1,000. ICC/IF: Use at 1:10-50. Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | Friend leukemia integration 1 transcription factor ERGB transcription factor FLI1 Fli-1 proto-oncogene Fli1 protooncogene Friend leukemia integration1 transcription factor Fli 1 proto oncogene |
|---|---|
| Target | FLI1 |
| Molecular Weight(MW) | 50.982 kDa (note) |
Application
Western blot analysis of FLI1 antibody (Center) (Cat.# AP6514c) in mouse lung tissue lysates(35ug/lane). FLI1 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of FLI1 Antibody (Center)(Cat#AP6514c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Application Notes | Recommended Starting Dilutions: WB: Use at 1:1,000. ICC/IF: Use at 1:10-50. Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
