Application
Western blot analysis of FLI1 antibody (N-term) in 293 cell line lysates (35ug/lane). FLI1 (arrow) was detected using the purified Pab.Application
FLI-1 Antibody (N-term) (Cat. #AP6514d) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FLI-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | FLI1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FLI1 (N-term K67)Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. |
| Immunogen | The immunogen corresponding to a region within amino acids 52 and 80 of human FLI1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Recommended Starting Dilutions: WB: Use at 1:000. IHC-P: Use at 1:50-100. Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Friend leukemia integration 1 transcription factor ERGB transcription factor FLI1 Fli-1 proto-oncogene Fli1 protooncogene Friend leukemia integration1 transcription factor Fli 1 proto oncogene |
|---|---|
| Target | FLI1 |
| Molecular Weight(MW) | 50.982 kDa (note) |
Application
Western blot analysis of FLI1 antibody (N-term) in 293 cell line lysates (35ug/lane). FLI1 (arrow) was detected using the purified Pab.
Application
FLI-1 Antibody (N-term) (Cat. #AP6514d) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FLI-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | Recommended Starting Dilutions: WB: Use at 1:000. IHC-P: Use at 1:50-100. Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
