Application
Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE Flightless I antibody diluted at 1:1000Application
Flightless I antibody [C2C3], C-term detects FLII protein at cytoplasm by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLII protein stained by Flightless I antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
Flightless I antibody [C2C3], C-term detects FLII protein by Western blot analysis. A. 50 µg rat muscle lysate/extract 5 % SDS-PAGE Flightless I antibody [C2C3], C-term dilution: 1:1000Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000| Product Name | Flightless I antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Flightless I (flightless I homolog (Drosophila))This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Flightless I. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, ChickenZebrafish (83%), Xenopus laevis (81%), Chicken (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLI antibody FLIL antibody Fli1 antibody MGC39265 antibody FLII antibody protein flightless-1 homolog antibody flightless I homolog (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 145 kDa (note) (4) |
| Cellular Localization | Nucleus (By similarity) , Cytoplasm , cytoskeleton (By similarity) , Centrosome (By similarity) |
Application
Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE Flightless I antibody diluted at 1:1000
Application
Flightless I antibody [C2C3], C-term detects FLII protein at cytoplasm by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLII protein stained by Flightless I antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Flightless I antibody [C2C3], C-term detects FLII protein by Western blot analysis. A. 50 µg rat muscle lysate/extract 5 % SDS-PAGE Flightless I antibody [C2C3], C-term dilution: 1:1000
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000| Positive Control | Raji , mouse brain , rat muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
