Application
Western blot analysis of FLIP in HeLa (left), Jurkat (middle) and K562 (right) cell lysates.| Product Name | FLIP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to c-FLIPc-FLIP was cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH AND CLARP. c-FLIP contains two death effector domains and has sequence homology to caspase-8. c-FLIP interacts with adapter protein FADD and caspaser-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Two splice variants of c-FLIP have been identified and termed FLIPl and FLIPs |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Western blot analysis (0.5-4 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually. The immunoaffinity-purified antibody detects 58 kDa FLIPL on SDS-PAGE immunoblots, in samples from human, mouse and rat origins. The antibody recognizes only the FLIPL form.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | I-FLICE CASP8AP1 CASH c-FLIPL Casper cFLIP Usurpin cFLIPR FLAME1 CFLAR CLARP cFLIPL c-FLIPR c-FLIPS c-FLIP MRIT FLAME cFLIPS IFLICE c FLIP I FLICE c FLIPL c FLIPR c FLIPS |
|---|---|
| Target | c-FLIP |
| Tissue Specificity | This antibody is specific for c-FLIP. |
Application
Western blot analysis of FLIP in HeLa (left), Jurkat (middle) and K562 (right) cell lysates.| Application Notes | Western blot analysis (0.5-4 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually. The immunoaffinity-purified antibody detects 58 kDa FLIPL on SDS-PAGE immunoblots, in samples from human, mouse and rat origins. The antibody recognizes only the FLIPL form.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C.The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
