Application
A. Western blot analysis on K562 cell lysate using anti-Flotillin-1 RabMAb (cat. # ) dilution 1:500| Product Name | Flotillin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP446Y] to Flotillin-1Flotillin-1, also known as Reggie-2, belongs to a larger family of proteins that share an evolutionarily conserved stomatin/prohibitin/flotillin/HflK/C (SPFH) domain . Flotillin-1 is a lipid raft-associated protein that has been implicated in various cellular processes. Flotillin was first found in cells that lack morphologically definable caveolae, as well as in non-caveolar membranes and was subsequently identified in low density detergent-insoluble complexes of endothelial cells, where they were termed flotillins . As well as its postulated role in neuronal regeneration, flotilllin-1 has been implicated in insulin signaling to trigger glucose transporter redistribution in adipocytes Flotillin also associates with phagosomes in macrophages . |
| Immunogen | A synthetic peptide corresponding to residues in human Flotillin-1 was used as immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP446Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | Flotillin1 Flotillin 1 |
|---|---|
| Target | Flotillin-1 |
| Molecular Weight(MW) | 48 (note) |
Application
A. Western blot analysis on K562 cell lysate using anti-Flotillin-1 RabMAb (cat. # ) dilution 1:500| Application Notes | For WB: Use at a dilution of 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
