Application
Immunofluorescence of FLT3 in human kidney tissue with FLT3 antibody at 20 ug/mL.Application
Western blot analysis of FLT3 in 3T3 cell lysate with FLT3 antibody at (A) 1 and (B)2 ug/mL.| Product Name | FLT3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FLT3This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015] |
| Immunogen | FLT3 antibody was raised against an 18 amino acid synthetic peptide near the center of human FLT3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB: 1 - 2 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | fms-related tyrosine kinase 3 FLK2 FLK-2 STK1 CD135 |
|---|---|
| Target | FLT3 |
| Tissue Specificity | At least three isoforms of FLT3 are known to exist; this antibody will detect all isoforms. |
Application
Immunofluorescence of FLT3 in human kidney tissue with FLT3 antibody at 20 ug/mL.
Application
Western blot analysis of FLT3 in 3T3 cell lysate with FLT3 antibody at (A) 1 and (B)2 ug/mL.| Application Notes | WB: 1 - 2 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
