Application
FLT3 antibody detects FLT3 protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. FLT3 antibody dilution: 1:500.| Product Name | FLT3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FLT3 (fms-related tyrosine kinase 3)This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FLT3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, DogHuman (100%), Rat (89%), Dog (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD135 FLK2 FLK-2 fms-related tyrosine kinase 3 STK1 |
|---|---|
| Target | FLT3 |
| Molecular Weight(MW) | 113 kDa (note) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
FLT3 antibody detects FLT3 protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. FLT3 antibody dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
