Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE Flotillin-2 antibody diluted at 1:1000Application
FMO1 antibody [N2C1], Internal detects FMO1 protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20℃ 100% MeOH for 5 min. Green: FMO1 protein stained by FMO1 antibody [N2C1], Internal diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | FMO1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FMO1 (flavin containing monooxygenase 1)Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FMO1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rabbit, Rhesus Monkey, BovineMouse (84%), Rat (84%), Dog (88%), Pig (88%), Rabbit (84%), Rhesus Monkey (98%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FMO1 antibody dimethylaniline oxidase 1 antibody fetal hepatic flavin-containing monooxygenase 1 antibody dimethylaniline monooxygenase [N-oxide-forming] 1 antibody FMO 1 antibody Flavin-containing monooxygenase 1 (fetal liver) antibody flavin contai |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (5) |
| Cellular Localization | Microsome membrane , Endoplasmic reticulum membrane |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE Flotillin-2 antibody diluted at 1:1000
Application
FMO1 antibody [N2C1], Internal detects FMO1 protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20℃ 100% MeOH for 5 min. Green: FMO1 protein stained by FMO1 antibody [N2C1], Internal diluted at 1:500. Blue: Hoechst 33343 staining.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
