Application
Immunofluorescence analysis of methanol-fixed A431, using FMO3 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using FMO3 antibody at 1:100 dilution.| Product Name | FMO3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FMO3 (flavin containing monooxygenase 3)The mammalian flavin-containing monooxygenases (FMO; EC 1.14.13.8) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme (Ziegler, 1990 [PubMed 2203193]; Hines et al., 1994 [PubMed 8128486]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FMO3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Rabbit, Rhesus Monkey, Chimpanzee, BovineRat (81%), Dog (84%), Rabbit (81%), Rhesus Monkey (93%), Chimpanzee (100%), Bovine (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FMOII antibody MGC34400 antibody TMAU antibody dJ127D3.1 antibody FMO3 antibody dimethylaniline oxidase 3 antibody FMO II antibody FMO 3 antibody hepatic flavin-containing monooxygenase 3 antibody dimethylaniline monooxygenase [N-oxide-forming] 3 |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (5) |
| Cellular Localization | Microsome membrane , Endoplasmic reticulum membrane |
Application
Immunofluorescence analysis of methanol-fixed A431, using FMO3 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using FMO3 antibody at 1:100 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
