Application
FMRP antibody detects FMR1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE FMRP antibody dilution: 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using FMRP antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using FMRP antibody at 1:100 dilution.| Product Name | FMRP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FMRP (fragile X mental retardation 1)The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FMRP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, BovineMouse (98%), Zebrafish (82%), Xenopus laevis (91%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FMRP antibody FRAXA antibody MGC87458 antibody POF antibody POF1 antibody FMR1 antibody fragile X mental retardation 1 protein antibody protein FMR-1 antibody fragile X mental retardation 1 antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (6) |
| Cellular Localization | Cytoplasm , Nucleus , nucleolus |
Application
FMRP antibody detects FMR1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE FMRP antibody dilution: 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using FMRP antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using FMRP antibody at 1:100 dilution.| Positive Control | HepG2 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
