Application
Western blot analysis of extracts from 3T3 and PC12 cells using FKHR(Ab-319) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | FOXO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FOXO1Transcription factor. |
| Immunogen | Peptide sequence around aa. 317~321 (T-S-S-N-A) derived from Human FKHR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FKH1, FKHR, FOXO1A, FOXO1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma, forkhead box protein O1, forkhead box protein O1A, forkhead box O1 |
|---|---|
| Target | FOXO1 |
| Molecular Weight(MW) | 78-82 (note) |
Application
Western blot analysis of extracts from 3T3 and PC12 cells using FKHR(Ab-319) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
