Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXO1 antibody at 1:100 dilution.| Product Name | FOXO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human FKHRFKHR-14E4 recognizes FKHR (ForKHead in Rhabdomyosarcoma), a 70a protein, which is one member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. |
| Immunogen | Amino acids 242-655 of FKHR expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 14E4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For WB: Use at a concentration of 0.1 - 2.0 μg/ml. Predicted molecular weight: 70 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 2308 Q12778 136533 FKHR FKH1 FOXO1A FOXO1 |
|---|---|
| Target | FKHR |
| Light Chain Type | kappa |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXO1 antibody at 1:100 dilution.| Positive Control | Cos 1 cells transfected with human FKHR |
|---|---|
| Application Notes | For WB: Use at a concentration of 0.1 - 2.0 μg/ml. Predicted molecular weight: 70 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
