Application
Immunohistochemical analysis of paraffin-embedded Colon ca, using FOXO1 antibody at 1:50 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXO1 antibody at 1:100 dilution.| Product Name | FOXO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [7H3] to FOXO1AFKHR-7H3 recognizes FKHR (ForKHead in Rhabdomyosarcoma), a 70a protein, which is a member of the forkhead homeotic gene family of transcription factors (FKH/HNF-3). Members of the forkhead family can be classified and grouped by their degree of homology within the 100-120aa region that is necessary and sufficient for DNA binding and binding-site specificity. The FKHR subfamily of forkhead proteins is relatively divergent from the other members of the forkhead transcription factor family. With all members in this subfamily containing a signature insertion of 5 amino acids next to the helix 3 in the middle of the forkhead domain, and lack the well conserved amino terminal KPPY sequence found in most forkhead genes. Recently, it has been shown that members of the FKHR gene family are phosphorylated by Akt and this phosphorylation regulates its cellular localization promoting cell survival. |
| Immunogen | Amino acids 242-655 of FKHR expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7H3 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| For WB: Use at a concentration of 0.1 - 2.0 µg/ml. Predicted molecular weight: 70 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 2308 Q12778 136533 FKHR FKH1 FOXO1A FOXO1 |
|---|---|
| Target | FKHR |
| Molecular Weight(MW) | 70 kDa (note) |
| Light Chain Type | kappa |
Application
Immunohistochemical analysis of paraffin-embedded Colon ca, using FOXO1 antibody at 1:50 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXO1 antibody at 1:100 dilution.| Positive Control | Cos 1 cells transfected with human FKHR |
|---|---|
| Application Notes | For WB: Use at a concentration of 0.1 - 2.0 µg/ml. Predicted molecular weight: 70 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
