Application
Western blot analysis of extracts from NIH/3T3 cells.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.| Product Name | FOXO3A (phospho Ser253) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FOXO3A (phospho Ser253)This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq] |
| Modification | p-Ser253 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AF6q21 DKFZp781A0677 FKHRL1 FKHRL1P2 MGC12739 MGC31925 |
|---|---|
| Target | FOXO3A (phospho Ser253) |
| Cellular Localization | Translocates to the nucleus upon oxidative stress and in the absence of survival factors. |
Application
Western blot analysis of extracts from NIH/3T3 cells.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
