Rabbit polyclonal antibody to FOXO3ATranscriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
Immunogen
Peptide sequence around aa.251~255(A-V-S-M-D) derived from Human FKHRL1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
AF6q21 DKFZp781A0677 FKHRL1 FKHRL1P2 FOXO2 FOXO3A MGC12739 MGC31925 FOXO3 forkhead in rhabdomyosarcoma-like 1 forkhead box protein O3 forkhead homolog (rhabdomyosarcoma) like 1 forkhead Drosophila homolog of in rhabdomyosarcoma-like 1 fork
Target
FOXO3A
Molecular Weight(MW)
97 (note)
Application
Application
Western blot analysis of extracts from 3T3, MCF and 293 cells using FKHRL1(Ab-253) Antibody
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.