Application
Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using FOXO3A antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FOXO3A antibody immunoprecipitates FOXO3A protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 μg of FOXO3A antibody 5% SDS-PAGE The immunoprecipitated FOXO3A protein was detected by FOXO3A antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXO3A protein stained by FOXO3A antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | FOXO3A antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FOXO3A (forkhead box O3)This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXO3A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Sheep, BovineRat (100%), Pig (100%), Sheep (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AF6q21 antibody DKFZp781A0677 antibody FKHRL1 antibody FKHRL1P2 antibody FOXO2 antibody FOXO3A antibody MGC12739 antibody MGC31925 antibody FOXO3 antibody forkhead in rhabdomyosarcoma-like 1 antibody forkhead box protein O3 antibody forkhead hom |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytosol , Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using FOXO3A antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FOXO3A antibody immunoprecipitates FOXO3A protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 μg of FOXO3A antibody 5% SDS-PAGE The immunoprecipitated FOXO3A protein was detected by FOXO3A antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXO3A protein stained by FOXO3A antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , Mouse brain , C2C12 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
