Application
Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3)Application
Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3).| Product Name | FOXP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [3G3] to FoxP3FoxP3 (Forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.Entrez Gene (mouse): X 2.1 cM, (human): Xp11.23 |
| Immunogen | Full-length His-tagged recombinant murine FoxP3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3G3 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| FACS: Note:Staining method: - Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. in the dark and RT. Wash. - Add cold fixation buffer diluted to working concentration (if commercial reagent is used, follow the manufacturer’: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | AIID DIETER IPEX JM2 PIDX XPID |
|---|---|
| Target | FoxP3 |
| Tissue Specificity | The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor, which is the master regulator in the development and function of regulatory T cells. |
Application
Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3)
Application
Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3).| Application Notes | FACS: Note:Staining method: - Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. in the dark and RT. Wash. - Add cold fixation buffer diluted to working concentration (if commercial reagent is used, follow the manufacturer’: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
