Application
FOXP3 antibody [C3], C-term detects FOXP3 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXP3 protein stained by FOXP3 antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
FOXP3 antibody [C3], C-term immunoprecipitates FOXP3 protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of FOXP3 protein by 4 μg FOXP3 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated FOXP3 protein was detected by FOXP3 antibody [C3], C-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000| Product Name | FOXP3 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Foxp3 (forkhead box P3)Probable transcription factor. Plays a critical role in the control of immune response. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse FOXP3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Bovine, Cat, Pig, Rat, Rhesus Monkey, SheepRat (92%), Cat (83%), Pig (83%), Sheep (83%), Rhesus Monkey (83%), Bovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | scurfy antibody forkhead box protein P3 antibody JM2 antibody scurfin antibody sf antibody Foxp3 antibody forkhead box P3 antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
FOXP3 antibody [C3], C-term detects FOXP3 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXP3 protein stained by FOXP3 antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
FOXP3 antibody [C3], C-term immunoprecipitates FOXP3 protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of FOXP3 protein by 4 μg FOXP3 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated FOXP3 protein was detected by FOXP3 antibody [C3], C-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000| Positive Control | mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
