Application
HEK293T cells transfected with either pCMV6-ENTRY FRKApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FRKmouse monoclonal antibody. ( Dilution 1:50)Application
Anti-FRK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with FRKApplication
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FRKmouse monoclonal antibody. ( Dilution 1:50)| Product Name | FRK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5G4] to FRKPositively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor. |
| Immunogen | Protein expressed in 293T cell transfected with human FRK expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5G4 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PTK5 GTK FRK RAK |
|---|---|
| Target | FRK |
| Tissue Specificity | is specific for human FRK. |
Application
HEK293T cells transfected with either pCMV6-ENTRY FRK
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FRKmouse monoclonal antibody. ( Dilution 1:50)
Application
Anti-FRK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with FRK
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FRKmouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
