Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FTO antibody diluted at 1:1000.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FTO antibody diluted at 1:1000.| Product Name | FTO antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FTO (fat mass and obesity associated)The exact function of this gene is not know. Studies in mice suggest that it may be involved in nucleic acid demethylation, and that its mRNA level is regulated by feeding and fasting. Genomewide association studies of type 2 diabetes indicate this gene as a diabetes susceptibility locus. Mutation in this gene has been associated with growth retardation, developmental delay, coarse facies, and early death. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FTO. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, RabbitMouse (82%), Rat (83%), Pig (85%), Rabbit (87%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | fat mass and obesity associated |
|---|---|
| Target | FTO |
| Molecular Weight(MW) | 58 kDa (note) |
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FTO antibody diluted at 1:1000.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FTO antibody diluted at 1:1000.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , 293T , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
